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Interfacial rheology as the effective tool to description of interfacial behaviour of biofilms
Kachlířová, Helena ; Kráčalík, Milan (referee) ; Smilek, Jiří (advisor)
The aim of this diploma thesis is to optimize a method of interfacial rheology for testing the interfacial behaviour of biofilms on the liquid-air interface and after that use the method for studying the biofilm formation under optimal and stress conditions. For studying the biofilm formation, Kombucha was used. It is a microbial culture forming a cellulose biofilm on the interface. As the stress conditions, reduction of sucrose concentration, change of pH and change of ionic strength was used. Next, the ability of regeneration of biofilm formed on the interface was studied. The biofilm formation was occured in all cases except of increasing ionic strength. As expected, the best biofilm biofilm growth was observed under optimal condition, which means a sucrose concentration 100 g/l.
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Overview of methodological approaches leading to qualitative anad quantitative microbial biofilm evaluation
Holmanová, Pavlína ; Konečná, Klára (advisor) ; Vejsová, Marcela (referee)
Charles Univerzity Faculty of Pharmacy in Hradec Králové Study program: Medical Laboratory Technician Author: Pavlína Holmanová Supervisor: RNDr. Klára Konečná, Ph.D. Title of bachelor thesis: Overview of methodological approaches leading to qualitative and quantitative microbial biofilm evaluation Background: The aim of this work was to compose an overview of methodical approaches leading to qualitative and quantitative evaluation of microbial biofilms. To optimize the methods leading to preparation of mounts with biofilm-forming microorganisms evaluated by epifluorescence or confocal laser scanning microscopy. Methods: Optimization of microbial biofilm staining procedures for subsequent evaluation by epifluorescence microscopy. Formation of microbial biofilms - cultivation of microorganisms in various cultivation media in a microtiter plate, preparation of solutions with fluorescent dyes. Observation of coloured, dispersed biofilms by fluorescence microscope. Results: Using fluorescence microscopy, optimal target concentrations of fluorescent dyes in various combinations were verified. The combination of Syto 9 (SYTO™ 9 Green Fluorescent Nucleic Acid Stain) and propidium iodide was confirmed to be effective for the determination of viability of biofilm-forming microbial cells. The combination of...
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Interfacial rheology as the effective tool to description of interfacial behaviour of biofilms
Kachlířová, Helena ; Kráčalík, Milan (referee) ; Smilek, Jiří (advisor)
The aim of this diploma thesis is to optimize a method of interfacial rheology for testing the interfacial behaviour of biofilms on the liquid-air interface and after that use the method for studying the biofilm formation under optimal and stress conditions. For studying the biofilm formation, Kombucha was used. It is a microbial culture forming a cellulose biofilm on the interface. As the stress conditions, reduction of sucrose concentration, change of pH and change of ionic strength was used. Next, the ability of regeneration of biofilm formed on the interface was studied. The biofilm formation was occured in all cases except of increasing ionic strength. As expected, the best biofilm biofilm growth was observed under optimal condition, which means a sucrose concentration 100 g/l.
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Pathogenesis of infectious diseases caused by biofilm forming microorganisms
Diepoltová, Adéla ; Konečná, Klára (advisor) ; Jílek, Petr (referee)
Charles Univerzity Faculty of Pharmacy in Hradec Králové Study program: Medical Laboratory Technician Author: Adéla Diepoltová Supervisor: RNDr. Klára Konečná, Ph.D. Title of bachelor thesis: Pathogenesis of infectious diseases caused by biofilm-forming microorganisms Background: The aim of this thesis is to describe the importance of microbial biofilms in medicine and in pathogenesis of infectious diseases. This thesis includes specification of the concept of biofilm, its structure and chemical composition, formation, development and processes occurring in biofilm which are directly related to pathogenesis of infectious diseases. Briefly, the most common infectious diseases associated with the formation of microbial biofilms are also mentioned. Main findings: Microbial biofilms are active layers of microorganisms and their metabolic products. Biofilm, unlike the planktonic phase, is sessile form of life. The microorganism first adheres to suitable substrate, then multiplies and produces metabolites, that create slime called matrix. Parts of the biofilm layer can tear off and colonize new environments. These environments include any natural niches with water access, industrial pipelines but host tissues, as well. Biofilms cause diseases such as otitis media, endocarditis, or pneumonia in patients...
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